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Data acquisition and control system for multiparameter cell sorting based on DEC LSI-11 microprocessor

L Voet, F J Kurmann, K Hannig

    Cytometry
    |May 1, 1982
    PubMed
    Summary
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    A new computer system enhances multiparameter flow cytometer/sorters with data acquisition, display, storage, and processing. This system enables rapid, efficient data handling for advanced cell analysis and sorting.

    Area of Science:

    • Biotechnology
    • Computer Science
    • Analytical Chemistry

    Background:

    • Flow cytometry and cell sorting are crucial for biological research.
    • Existing systems often lack integrated data processing and high-speed acquisition capabilities.
    • Advanced computational tools are needed to maximize the utility of multiparameter flow cytometers.

    Purpose of the Study:

    • To develop a versatile computer system for multiparameter flow cytometer/sorters.
    • To integrate data acquisition, display, storage, and processing functionalities.
    • To enhance cell sorting control and data analysis efficiency.

    Main Methods:

    • Implementation of a computer system with Direct Memory Access (DMA) for data acquisition.
    • Software-based multichannel analyzer features using standard interface boards.

    Related Experiment Videos

  • Event-by-event data storage in list-mode with 12-bit resolution.
  • Processor control for cell sorting operations.

    • Main Results:

    • The system supports one or two parameters in histogram mode and multiple parameters in list-mode.
    • Achieved data acquisition rates exceed 8000 events/sec for eight-parameter handling.
    • Data acquisition deadtime is minimized and largely independent of software routines.
    • The system demonstrates capacity for rapid data acquisition and efficient process control.

    Conclusions:

    • The developed computer system significantly expands the capabilities of multiparameter flow cytometers.
    • It offers high-speed data acquisition and processing, crucial for advanced cell analysis.
    • The system's flexibility and efficiency pave the way for more sophisticated flow cytometry applications.