Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Paulomycins A and B. Isolation and characterization

A D Argoudelis, T A Brinkley, T F Brodasky

    The Journal of Antibiotics
    |March 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Controlling a new plasma regime.

    Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2024
    Same author

    Syntheses of benzoquinolizidine and benzoindolizidine derivatives as anti-amnesic agents.

    Bioorganic & medicinal chemistry·1999
    Same author

    Syntheses of 1,2-diamino and 1,2-aminoalcohol derivatives in the piperidine and pyrrolidine series as anti-amnesic agents.

    Bioorganic & medicinal chemistry·1999
    Same author

    Isolation and identification of a major metabolite of PNU-107859, an MMP inhibitor from the biliary fluid of rats.

    Journal of pharmaceutical sciences·1999
    Same author

    Solution structures of stromelysin complexed to thiadiazole inhibitors.

    Protein science : a publication of the Protein Society·1998
    Same author

    In vitro metabolic transformations of 2,4-dipyrrolidinylpyrimidine: a chemical probe for P450-mediated oxidation of tirilazad mesylate.

    Xenobiotica; the fate of foreign compounds in biological systems·1997

    Two novel antibiotics, paulomycin A and B, were identified from Streptomyces paulus. These compounds exhibit significant activity against Gram-positive bacteria due to their unique isothiocyanate group.

    Area of Science:

    • Microbiology
    • Natural Products Chemistry
    • Pharmacology

    Background:

    • Streptomyces paulus strain 273 produces bioactive compounds.
    • Antibiotics are crucial for combating bacterial infections.
    • The isothiocyanate functional group is found in various bioactive molecules.

    Purpose of the Study:

    • To characterize paulomycin A and paulomycin B.
    • To investigate the antibacterial spectrum of these novel antibiotics.
    • To explore the structural relationship with other isothiocyanate-containing antibiotics.

    Main Methods:

    • Isolation and purification of paulomycin A and B from Streptomyces paulus culture.
    • Determination of chemical structures using spectroscopic methods (e.g., NMR, Mass Spectrometry).

    Related Experiment Videos

  • Antibacterial activity assays against a panel of Gram-positive bacteria.

    • Main Results:

    • Paulomycin A (C34H46N2O17S) and paulomycin B (C33H44N2O17S) were successfully isolated and characterized.
    • Both antibiotics demonstrated notable activity against various Gram-positive bacterial species.
    • The presence of an isothiocyanate group was confirmed in both compounds.

    Conclusions:

    • Paulomycin A and B represent a new class of antibiotics with potential therapeutic applications.
    • Their structural similarity to senfolomycins and proceomycin highlights a conserved pharmacophore.
    • Further research is warranted to explore their mechanism of action and optimize their efficacy.