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Heme biosynthesis in the heart

R Sedman, G Ingall, G Rios

    Biochemical Pharmacology
    |March 1, 1982
    PubMed
    Summary

    Fasting significantly reduces heme synthesis in rat hearts by decreasing delta-aminolevulinic acid synthetase activity. This enzyme

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    Area of Science:

    • Biochemistry
    • Cardiovascular Physiology
    • Metabolic Regulation

    Background:

    • Heme biosynthesis is crucial for cardiac energy metabolism.
    • The regulation of heme synthesis in the heart is not fully understood.

    Purpose of the Study:

    • To investigate the regulation of heme a and heme b biosynthesis in the rat heart.
    • To determine the role of delta-aminolevulinic acid synthetase in cardiac heme production.

    Main Methods:

    • Isolated heart perfusion system in fed and fasted rats.
    • Measurement of heme a and heme b synthesis rates using [14C] glycine incorporation.
    • Assay of delta-aminolevulinic acid synthetase activity.
    • Effect of cobalt on enzyme activity in vitro.

    Main Results:

    • Fasting decreased delta-aminolevulinic acid synthetase activity to 30% in rat hearts.
    • [14C] Glycine incorporation into heme a and b was reduced by 70% in fasted rats.
    • Cobalt addition decreased enzyme activity, mimicking in vivo effects.

    Conclusions:

    • Delta-aminolevulinic acid synthetase activity is a key regulator of heme a and b synthesis in the heart.
    • Fasting profoundly impacts cardiac heme biosynthesis pathways.
    • The isolated heart perfusion system is a viable model for studying cardiac metabolic regulation.

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