Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coulometric microrespirometry

A A Heusner, J P Hurley, R Arbogast

    The American Journal of Physiology
    |July 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a sensitive capacitive coulometry device for measuring oxygen consumption in closed systems. The microrespirometer offers precise O2 measurements suitable for various biological applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Rapid pre-concentration of mercury in solids and water for isotopic analysis.

    Analytica chimica acta·2019
    Same author

    Understanding STAT3 signaling in cardiac ischemia.

    Basic research in cardiology·2016
    Same author

    Understanding Complete Pathologic Response in Oesophageal Cancer: Implications for Management and Survival.

    Gastroenterology research and practice·2015
    Same author

    The 100 most cited publications in cardiac surgery: a bibliometric analysis.

    Irish journal of medical science·2014
    Same author

    [In memoriam Prof. em. Dr. med. Ernst Kern].

    Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen·2014
    Same author

    Influences of watershed characteristics on mercury levels in wisconsin rivers.

    Environmental science & technology·2011

    Area of Science:

    • Physiology
    • Biochemistry
    • Microbiology

    Background:

    • Metabolic studies require precise measurement of oxygen (O2) consumption.
    • Existing methods may lack the sensitivity or precision for certain biological applications.

    Purpose of the Study:

    • To develop and validate a novel microrespirometer utilizing capacitive coulometry for O2 consumption measurements.
    • To assess the accuracy, precision, and operational range of the developed device.

    Main Methods:

    • Capacitive coulometry principle: automatic quantitative replacement of consumed O2 with electrolytic O2.
    • Device operation: discharging a capacitor through a CuSO4 solution to produce O2.
    • Autoclavable design for sterile applications.

    Related Experiment Videos

    Main Results:

    • High sensitivity: better than 0.1 nl O2 detection.
    • Accuracy and precision: within 1% for 1 nl O2 volume across a wide temperature range (2.8–40°C).
    • Measurement range: upper limit of 1 ml/h O2 consumption.

    Conclusions:

    • The microrespirometer is highly sensitive, accurate, and precise for O2 consumption studies.
    • Its autoclavable nature makes it ideal for sterile metabolic research in microbiology and cell cultures.
    • The technique is well-suited for comparative physiology and other biological research requiring precise respirometry.