New method for hemoglobin determination by using sodium lauryl sulfate (SLS)

Area of Science:

• Clinical Chemistry
• Biochemistry
• Analytical Chemistry

Background:

• Hemoglobin (Hb) determination is crucial in clinical diagnostics.
• Current methods often involve toxic reagents like KCN and NaN3, posing environmental risks.
• There is a need for safer and eco-friendly Hb assay methods.

Purpose of the Study:

• To introduce a new, environmentally friendly method for hemoglobin determination.
• To utilize sodium lauryl sulfate (SLS) as a primary reagent for Hb analysis.
• To establish the SLS-methemoglobin (SLS-Hb) method as a viable alternative for clinical laboratories.

Main Methods:

• Development of a novel hemoglobin assay using sodium lauryl sulfate (SLS).
• Investigation of SLS's presumed oxidative activity on hemoglobin intermediates.
• Adaptation of the SLS-Hb method for both manual procedures and automated analyzers (Hemalog-8/90).

Main Results:

• The SLS-methemoglobin (SLS-Hb) method successfully determines hemoglobin levels.
• This method eliminates the need for external oxidative reagents.
• The procedure avoids the generation of hazardous waste products (KCN, NaN3).

Conclusions:

• The developed SLS-methemoglobin (SLS-Hb) method provides an eco-friendly alternative for hemoglobin quantification.
• This method is applicable in routine clinical laboratory settings, enhancing safety and reducing environmental impact.
• Further research is needed to fully elucidate the oxidative mechanism of SLS on hemoglobin.