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Rapid screening test for sulfamethazine in swine feeds
Journal - Association of Official Analytical Chemists
|May 1, 1982
Summary
A rapid 10-minute field test detects sulfamethazine (SM) in swine feed. This simple method identifies feed contamination, helping prevent violative residues in pork.
Area of Science:
- Veterinary Science
- Analytical Chemistry
- Food Safety
Background:
- Sulfamethazine (SM) is an antibiotic used in swine.
- Ensuring feed safety and preventing violative residues in meat is crucial for public health and regulatory compliance.
- Current methods for SM detection may not be suitable for rapid field screening.
Purpose of the Study:
- To develop and describe a simple, rapid, qualitative screening test for sulfamethazine in swine feed.
- To enable field testing of feed for SM contamination.
- To aid in the detection of cross-contaminated withdrawal feeds.
Main Methods:
- A 10-minute, two-tube qualitative screening test utilizing alumina and anion exchange resins.
- The method involves sample loading, solvent percolation, and reaction with Bratton-Marshall reagents for colorimetric detection.
- Optional dye concentration step for lower detection limits and permanent color standards are described.
Main Results:
- The test can detect SM in feed down to 0.15 ppm.
- An optional dye concentration step allows detection of 0.02 ppm SM.
- The method can identify cross-contaminated feeds with greater than 2 ppm SM, relevant for preventing violative liver residues.
Conclusions:
- A simple, field-deployable qualitative test for SM in swine feed has been developed.
- This method can help identify contaminated feed, reducing the risk of violative SM residues in swine.
- The test is specific for amphoteric primary aromatic amino-containing compounds with a pKa near SM.