Related Experiment Video
Updated: Aug 19, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
Physico-chemical evidence for the interaction between aldolase and glyceraldehyde-3-phosphate dehydrogenase
Abstract:
Polarization of fluorescence measurements of aldolase and D-glyceraldehyde-3-phosphate dehydrogenase labeled with fluorescein isothiocyanate have been used to detect the possible formation of a soluble complex between the proteins. The results suggest an interaction between aldolase and D-glyceraldehyde-3-phosphate dehydrogenase with an apparent dissociation constant 3 X 10(-7) M and an apparent stoichiometry of two aldolase tetramers bound per tetramer of D-glyceraldehyde-3-phosphate dehydrogenase.
Related Concept Videos
Energy-requiring Steps of Glycolysis
Glycolysis: Preparatory Phase
Base-Catalyzed Aldol Addition Reaction
Dehydration of Aldols to Enals: Base-Catalyzed Aldol Condensation
C–C Bond Formation: Aldol Condensation Overview
Acid-Catalyzed Aldol Addition Reaction

