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Related Experiment Videos

A new enzymatic method to determine creatine

M Yasuhara, S Fujita, K Arisue

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |July 1, 1982
    PubMed
    Summary
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    A novel enzymatic assay accurately determines creatine levels in serum and urine within 20 minutes. This method utilizes specific enzymes for precise creatine measurement, offering a rapid and reliable diagnostic tool.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry
    • Enzymology

    Background:

    • Accurate creatine measurement is crucial for diagnosing various medical conditions.
    • Existing methods for creatine determination may be time-consuming or less sensitive.

    Purpose of the Study:

    • To develop and validate a new, rapid enzymatic method for creatine determination in biological samples.
    • To compare the performance of the new method against a standard assay.

    Main Methods:

    • Enzymatic assay utilizing creatine amidinohydrolase, sarcosine oxidase, and formaldehyde dehydrogenase.
    • Measurement of NADH + H+ production at 340 nm.
    • Validation using standard solutions and comparison with the Folin method.

    Main Results:

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    • The assay is linear up to 50 mg/L for serum and 800 mg/L for urine.
    • High correlation coefficients (0.995 for serum, 0.994 for urine) were observed when compared to the Folin method.
    • The assay provides results in under 20 minutes.

    Conclusions:

    • The described enzymatic method offers a fast, accurate, and reliable approach for creatine quantification in serum and urine.
    • This method is suitable for routine clinical laboratory use.