Testosterone-dependent oxygen consumption in membrane vesicles of Pseudomonas testosteroni

Oxygen consumption was measured in membrane vesicles of Pseudomonas testosteroni using conditions similar to those identified for testosterone transport in these vesicles. Testosterone and NAD+, which are primary requirements for testosterone transport, were both required for maximum oxygen consumption suggesting that testosterone transport and oxygen consumption were linked. Testosterone-dependent oxygen consumption was inhibited by 95% by 1 mM KCN indicating that the electron-transport chain could be involved in this process. Respiration appears to play in important role in the transport of steroids by membrane vesicles of P. testosteroni.