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Membrane IgM of bovine lymphocytes
Veterinary Immunology and Immunopathology
|May 1, 1982
Summary
Bovine leukemia virus infection alters membrane immunoglobulins in calves. Membrane-bound IgM in infected calves shows a heavier mu-chain compared to serum IgM.
Area of Science:
- Veterinary Immunology
- Molecular Biology
- Virology
Background:
- Bovine leukemia virus (BLV) is an economically significant pathogen in cattle.
- Membrane immunoglobulins (mIg) play crucial roles in lymphocyte function and immune responses.
- Understanding alterations in mIg during viral infections is key to deciphering pathogenesis.
Purpose of the Study:
- To characterize the membrane immunoglobulins (mIg) on peripheral blood lymphocytes (PBL) from BLV-infected calves.
- To compare the molecular characteristics of mIg, specifically IgM, with those from healthy cattle.
Main Methods:
- Isolation and characterization of membrane immunoglobulins from PBL of four BLV-infected calves and one normal cow.
- Analysis of the mu-chain of membrane-bound IgM using apparent molecular weight (AMW) determination.
Main Results:
- Membrane immunoglobulins isolated consisted of IgM.
- The mu-chain of membrane-bound IgM exhibited an AMW of 95,000 daltons.
- This mu-chain was approximately 10,000 daltons heavier than the mu-chain of serum IgM.
Conclusions:
- The heavier molecular weight of the mu-chain is a characteristic of membrane-bound IgM in bovine origin.
- BLV infection may influence the post-translational modification or structure of membrane-bound IgM.