Related Experiment Videos
An improved short-term culture method for human mammary epithelial cells
Gan
|February 1, 1982
Abstract:
An improved short-term culture method for human mammary epithelial cells was developed and the details are presented. Briefly, after the digestion of biopsy tissues with 0.2% collagenase solution for 16 to 18 hr, 4 consecutive fractions were obtained by differential sedimentation. The last fraction, S-4, contained organoids having alveolar- or duct-like structures and gave rise to mammary epithelial cell growth with minimal contamination of fibroblasts.