Hereditary abnormality in tapetum lucidum of the Siamese cats. A histochemical and quantitative study

Gross examination showed a weaker reflection (less shining) of the tapetum lucidum of the Siamese cats compared with common cats. Toluidine blue sections revealed that many tapetal cells were weakly stained and giving vacuolated appearance under high magnification. Further examination with electron microscope showed that those weakly stained cells were filled with disrupted tapetal rods. In these affected cells, the arrangement of the tapetal rods was no longer regular. The membranes of the tapetal rods were either enlarged or disrupted. Some of them appeared to be myelin-like structures. The cores of the tapetal rods were either empty or filled with electron-dense materials which may be the remnant of the original cores. The severity of this type of abnormality or degeneration in the tapetum varied from layers to layers. Those layers closer to the retina showed a greater number of cells with degeneration. Quantitative analysis of histochemical detection of zinc showed a significantly smaller amount of zinc in tapetal rods of the Siamese cats as compared with common cats. Less zinc and disruption of the regular arrangement of the tapetal rods may result in weaker reflection of light by Siamese cat tapetum. In four of the nine Siamese cats studied, this type of abnormality was observed. It suggests that it is a hereditary disorder of relatively high frequency.