Adsorption of human alpha (leukocyte) interferon on glass: contributions of electrostatic and hydrophobic forces

Human leukocyte-derived interferon (HuIFN-alpha) can be displaced from controlled-pore glass (CPG) with alkylamines at 0.1-0.3 M concentration. The eluting power of an alkylamine correlates positively with the extent of its alkylation: CH3NH3 + less than (CH3)2NH2 + less than (CH3)3NH+ less than (CH3)4N+. Interferon can also be recovered using an electrolyte and ethylene glycol as cosolvents. Apparently, both hydrophobic and electrostatic forces are involved in the adsorption of HuIFN-alpha to CPG.