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Related Experiment Videos

Post mortem tissue levels of ascorbic acid in a scurvy case

W J Allender

    Journal of Analytical Toxicology
    |July 1, 1982
    PubMed
    Summary

    Severe Vitamin C deficiency, though rare, can be fatal. This study details a case in an infant and establishes normal Vitamin C levels using HPLC analysis.

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    Area of Science:

    • Biochemistry
    • Clinical Medicine
    • Analytical Chemistry

    Background:

    • Ascorbic acid (Vitamin C) deficiency exists on a spectrum from mild to life-threatening.
    • Profound deficiency is uncommon in humans, typically occurring under extreme physiological stress.

    Observation:

    • A rare case of profound ascorbic acid deficiency was identified in an infant.
    • Autopsy samples were analyzed to determine Vitamin C levels.

    Findings:

    • A sensitive amino-bonded reverse-phase high-performance liquid chromatographic (HPLC) technique was employed for precise Vitamin C quantification.
    • Comparison with control cases established average normal levels of Vitamin C in autopsy tissues.

    Implications:

    • This study provides a benchmark for normal Vitamin C levels, crucial for diagnosing deficiency.
    • The findings highlight the utility of HPLC in forensic and clinical pathology for assessing nutritional status.

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