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Related Experiment Videos

Enzymatic assay for cholesterol ester hydrolase activity

L L Gallo, R Atasoy, G V Vahouny

    Journal of Lipid Research
    |September 1, 1978
    PubMed
    Summary

    A new assay accurately measures cholesterol ester hydrolase (CEH) activity. This rapid enzymatic method simplifies cholesterol ester hydrolase activity determination, offering reproducible results.

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    Area of Science:

    • Biochemistry
    • Enzymology
    • Analytical Chemistry

    Background:

    • Cholesterol ester hydrolase (CEH) plays a crucial role in lipid metabolism.
    • Accurate measurement of CEH activity is essential for research and diagnostics.
    • Existing methods for CEH assay can be time-consuming or complex.

    Purpose of the Study:

    • To develop and validate a rapid and accurate assay for cholesterol ester hydrolase (CEH) activity.
    • To provide a reproducible method for quantifying CEH enzymatic function.

    Main Methods:

    • Enzyme-substrate incubation followed by isopropanol extraction.
    • Enzymatic determination of free cholesterol using cholesterol oxidase and peroxidase.
    • Spectrophotometric detection of a colored product at 500 nm.

    Main Results:

    • The assay provides rapid and accurate measurements of CEH activity.
    • The method demonstrates high reproducibility.
    • Results correlate well with established chromatographic radioassay techniques.

    Conclusions:

    • A novel, efficient enzymatic assay for cholesterol ester hydrolase activity has been established.
    • This method offers a reliable alternative for CEH activity determination.
    • The assay's speed and accuracy facilitate broader application in biochemical research.

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