[Dynamics of initial L-cell adhesion. I. The effect of serum and albumin]

The adhesion of neoplastic mouse fibroblasts of line L was investigated during the formation of initial cell-substrate contact after the settling of spherical cells from suspension. nu (t), part of cell population which remained attached to the substrate after the impulse action of detaching force in the moment t, is used as a quantitative measure of the initial attachment process. The effects of the detaching force value and of the experimental medium composition on the main parameters of sigmoidal function nu (t) are studied. It is shown that 1) with the change of the detachment force three main parameters of function nu (t) are changed; 2) with the increase of the concentration of serum added in the Eagle medium the cell attachment becomes monotonely weaker and slower; albumin has the similar effect.