[Stability and viability of spermatogenic cells after their separation and fractionation]

Spermatogenic cells isolated from seminiferous tubules by enzymatic treatment are very sensitive to the action of Triton X-100. The cells isolated by mechanical dissociation of spermatogenic epithelium are destroyed during storage. On the contrary, the cell separation by sedimentation velocity technique in human serum protein gradient leads to cell stabilization. As the result, the cells in collected fractions kept their living capacity and, what is more, their specific features.