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Related Experiment Videos

[Free iron in intracellular membrane structures]

N I Tarasova, O A Kovalenko, A F Vanin

    Biofizika
    |July 1, 1982
    PubMed
    Summary

    Long-term existence of liposomes loaded with bivalent iron was demonstrated. Heating and detergents destabilize these structures, releasing iron and forming nitrosyl complexes, indicating iron

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    Dinitrosyl Iron Complexes with Thiol-Containing Ligands Can Suppress Viral Infections as Donors of the Nitrosonium Cation (Hypothesis).

    Biophysics·2020

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biophysics

    Context:

    • Liposomes serve as model membrane systems.
    • Bivalent iron (ferrous iron) is a crucial biological element.
    • Understanding iron's intracellular localization is vital for cell function.

    Purpose:

    • To investigate the long-term stability of liposomes loaded with bivalent iron.
    • To examine the effects of heat and detergents on iron-loaded liposomes and cellular structures.
    • To determine the localization of free iron within animal liver cells.

    Summary:

    • Liposomes from egg yolk phospholipids were loaded with bivalent iron, showing long-term stability.
    • Similar effects of heating and detergents were observed on iron-loaded liposomes and cellular components (mitochondria, hepatocytes, liver).
    • These agents induced iron liberation and nitrosyl complex formation (complexes 2.03), suggesting membrane structure disruption.

    Impact:

    • Demonstrates that free iron in animal liver cells is associated with closed membrane structures.
    • Provides insights into the mechanisms of iron release and complex formation.
    • Highlights the utility of liposomes as model systems for studying intracellular iron dynamics.

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