Separation of human pro alpha 1 (I) and pro alpha 2 (I) procollagen chains by reverse phase high performance liquid...

Human pro alpha 1 (I) and pro alpha 2 (I) procollagen chains have been separated on a large por (33 nm) reverse phase high performance liquid chromatography C18 column using an acetonitrile aqueous gradient containing 9 mM trifluoroacetic acid. The separation of the chains was evaluated by gel electrophoresis. The yield of the chromatography, based on radiolabel recovery, is equal to or better than 80%. Absorbance monitoring at 210 nm permits detection of less than 5 micrograms procollagen.