[Haemagglutination inhibition test: improvements, limits and advantages of the method. Comparison with neutralization...

In this study, we report some technical improvements which enable us to envisage wider use of the haemagglutination test and haemagglutination inhibition of the rabies virus. Moderated trypsination of sensitive red blood cells and their counting are important parameters. An interesting application of the test was the haemagglutination competition enabling the amount of soluble glycoprotein (obtained during the fractionating of the virus components) to be evaluated. From antibody kinetics of vaccinated dogs, a study of the correlation which exists between the IHA titres, SN mice titres and SN-RFFIT titres was carried out. Satisfactory correlation was obtained between these three techniques.