The probable binding between cations and stimulatory protein kinase modulator and subsequent stimulation on...

The formation of complexes between cations and stimulatory protein kinase modulator (PKMs) were performed by preincubation and gel filtration. The stimulatory effects of Fe3+, Fe2+, Mg2+, and Co2+ complexes on cerebellar modulator-dependent protein kinases (M-PK) were similar to those without preformation of complexes by preincubation and gel filtration. Antagonism by Ca2+ on the stimulatory effect of Mg2+ was noted in spite of the ineffectiveness of Ca2+ when present alone.