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Mitosis in a cell with multiple centrioles

D Ring, R Hubble, M Kirschner

    The Journal of Cell Biology
    |September 1, 1982
    PubMed
    Summary
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    N115 neuroblastoma cells have many microtubule organizing centers (MTOCs) that organize microtubules during mitosis. Their centriole number varies at mitotic poles, questioning the centriole

    Area of Science:

    • Cell Biology
    • Cytoskeleton Dynamics
    • Mitosis

    Background:

    • Microtubule organizing centers (MTOCs) are crucial for cell division.
    • N115 mouse neuroblastoma cells are known to possess numerous MTOCs, identified as single centrioles.
    • Understanding MTOC behavior during the cell cycle is key to comprehending cell division fidelity.

    Purpose of the Study:

    • To investigate the distribution and activity of multiple MTOCs in N115 cells throughout the cell cycle.
    • To determine the role of numerous centrioles in establishing bipolar mitosis in N115 cells.

    Main Methods:

    • Labeling microtubules using anti-tubulin antibodies.
    • Labeling chromatin with Hoechst dye (Bisbenzimid).
    • Ultrastructural identification of centrioles and observation of MTOC distribution during cell division.

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    Main Results:

    • Multiple MTOCs persist and actively organize microtubules during mitosis in N115 cells.
    • MTOCs exhibit dynamic movements: dispersed in prophase, clustered in prometaphase, and forming ring-shaped groups at poles in metaphase/anaphase.
    • N115 cells consistently display normal bipolar mitosis despite unequal centriole distribution at the poles.

    Conclusions:

    • The presence of numerous MTOCs does not disrupt the establishment of bipolar mitosis in N115 cells.
    • Centriole number at mitotic poles appears less critical for bipolar spindle formation than previously thought.
    • Further research is needed to elucidate the mechanisms ensuring bipolar division in cells with supernumerary centrioles.