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Immune response in experimentally induced uremia. IV. Characterization of suppressor peritoneal macrophages in the
Abstract:
The effect of unstimulated uremia PM phi on the response of syngeneic control NA spleen cells to mitogens was examined. Nonstimulated PM phi purified from uremic rats were found to be significantly more suppressive than similar numbers of control PM phi. It was found that indomethacin treatment, as well as anti-la treatment, only partially reverses the suppressive activity of uremic PM phi as compared to control PM phi, thus indicating that uremic suppressor PM phi have different characteristics from control PM phi. In addition, uremic PM phi suppress via a suppressor factor released to the supernatant over 24 hr incubation. The enhanced suppressor activity of uremic PN phi as well as their different characteristics may have relevance to the severely suppressed cell-mediated immunity observed in uremia.
Insights
Uremic macrophages (PM phi) from rats show enhanced immune suppression compared to normal macrophages. This heightened activity, linked to a suppressor factor, may explain reduced cell-mediated immunity in uremia.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Uremia is associated with suppressed cell-mediated immunity.
- The role of unstimulated macrophages in uremic immune dysfunction is not fully understood.
Purpose of the Study:
- To investigate the suppressive effects of unstimulated macrophages from uremic rats on immune responses.
- To characterize the nature of this enhanced suppression.
Main Methods:
- Purification of nonstimulated peritoneal macrophages (PM phi) from uremic and control rats.
- Assessment of PM phi suppressive activity on syngeneic spleen cell responses to mitogens.
- Evaluation of the effects of indomethacin and anti-Ia treatment on PM phi suppressive activity.
- Analysis of suppressor factor release by uremic PM phi.
Main Results:
- Nonstimulated macrophages from uremic rats exhibited significantly greater suppressive activity than those from control rats.
- Indomethacin and anti-Ia treatments only partially reversed the suppressive activity of uremic PM phi, suggesting distinct characteristics.
- Uremic PM phi release a suppressor factor into the supernatant during incubation.
Conclusions:
- Uremic macrophages possess enhanced suppressor activity compared to control macrophages.
- The enhanced suppression by uremic PM phi is mediated by a released suppressor factor and exhibits different characteristics from control PM phi.
- These findings may explain the suppressed cell-mediated immunity observed in uremia.