[Comparative study of the action of different antibiotics on the membrane dehydrogenase activity in Micrococcus...

The object of this work was to study the effect of antibiotics belonging to the groups of penicillin, tetracycline and aminoglycosides on the activity of lactate dehydrogenase, alcohol dehydrogenase and malate dehydrogenase in the membranes of Micrococcus lysodeikticus. Streptomycin, benzylpenicillin, carbenicillin and phenoxymethylpenicillin decreased the activity of the above dehydrogenases. Tetracycline and oxytetracycline activated lactate dehydrogenase and alcohol dehydrogenase in the membranes, but decreased their activity in the supernatant fraction of disintegrated membranes. The enzyme activity in the membranes was particularly inhibited by neomycin.