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Updated: Jul 21, 2026

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
Published on: February 15, 2012
[Fine structure of Bifidobacterium bifidum]
Abstract:
Cultures of Bifidobacterium bifidum 1 were grown in liquid and semisolid nutrient media for 14 hours to 6 days. Their preparations were then studied by electron microscopy on ultra-thin sections after a routine chemical fixation or on replicas after rapid freezing-fracturing and etching. This procedure made it possible to obtain new data about the structural organization of cells in the course of growth of cultures which were characterized by desynchronization of growth and division processes. The growth was inhibited later when populations reached the highest number of cells in the given set of conditions. The zone of nuclear vacuoles contained peculiar membrane structures which appeared and underwent certain changes related, apparently, to the mode of growth of this bacterium.
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