[Parameters of probe fluorescence spectra under model conditions]

An expression is presented which describes fluorescence spectra of 1-anilinonaphtaleno-8-sulfonate and N-phenyl-1-naphtilamine in the solvents of different polarity and viscosity. This expression allows homogeneous and nonhomogeneous components of the spectrum to be distinguished. Location of the centrum of nonhomogeneous broadening is shown to characterize better the hydrophobic surroundings, than the location of the spectrum maximum. A connection is found between the dispersion of nonhomogeneous broadening and viscosity of the solvent. The method proposed can be used for characterizing the homogeneous sites of the probe binding.