Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intercellular communication, three-dimensional cell contact and radiosensitivity

H Dertinger, G Hinz, K H Jakobs

    Biophysics of Structure and Mechanism
    |January 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mean inactivation dose: a useful concept for intercomparison of human cell survival curves. 1984.

    Radiation research·2012
    Same author

    Diffusion of Cs atoms in Ne buffer gas measured by optical magnetic resonance tomography.

    Optics express·2009
    Same author

    [WITHDRAWN Control of platelet function by Epac protein.]

    Der Urologe. Ausg. A·2007
    Same author

    Brefeldin A action and recovery in Chlamydomonas are rapid and involve fusion and fission of Golgi cisternae.

    Plant biology (Stuttgart, Germany)·2007
    Same author

    Renal mesangial cells: moving on sphingosine kinase-1.

    British journal of pharmacology·2007
    Same author

    Analgesic and disease modifying effects of interferential current in psoriatic arthritis.

    Rheumatology international·2006
    Same journal

    Transduction in photoreceptors: determination of the pigment transition or state coupled to excitation.

    Biophysics of structure and mechanism·2012
    Same journal

    Upper limit on translational diffusion of visual pigment in intact unfixed barnacle photoreceptors.

    Biophysics of structure and mechanism·2012
    Same journal

    Properties of the on-transient of the intracellular response in the barnacle photoreceptor.

    Biophysics of structure and mechanism·2012
    Same journal

    Absorption of light by metarhodopsin modifies the effect of a conditioning light on the barnacle photoreceptor.

    Biophysics of structure and mechanism·2012
    Same journal

    The effects of Mn2+ and Ca2+ on the prolonged depolarising after-potential in barnacle photoreceptor.

    Biophysics of structure and mechanism·2012
    Same journal

    Blue adaptation: an experimental tool for the study of visual receptor mechanisms and behaviour of Drosophila.

    Biophysics of structure and mechanism·2012
    See all related articles

    Mammalian cells in spheroids show reduced radiosensitivity compared to monolayers. This is linked to decreased adenylate cyclase activity and cell coupling in three-dimensional cultures, suggesting physiological changes impact radiation response.

    Area of Science:

    • Cell biology
    • Radiation biology
    • Biophysics

    Background:

    • Mammalian cells cultured as spheroids exhibit lower radiosensitivity than monolayer cultures.
    • Electrically coupled cells form gap junctions, influencing cellular communication and behavior.
    • Three-dimensional cell-cell contact in spheroids may alter cellular physiology.

    Purpose of the Study:

    • To investigate the role of cell coupling and three-dimensional contact in the reduced radiosensitivity of mammalian cell spheroids.
    • To explore the relationship between cell coupling, adenylate cyclase activity, and spheroid radiosensitivity.

    Main Methods:

    • Culturing mammalian cells as spherical clones (spheroids) and monolayers.
    • Measuring cell coupling using gap junction activity.

    Related Experiment Videos

  • Assessing adenylate cyclase activity in both spheroids and monolayers.
  • Correlating cell coupling and cyclase activity with radiosensitivity.

    • Main Results:

    • A correlation was observed between cell coupling and adenylate cyclase activity in monolayers.
    • Adenylate cyclase activity significantly dropped in spheroids composed of coupled cells compared to monolayers.
    • Cell coupling decreased with spheroid age or maturation.

    Conclusions:

    • Profound physiological alterations occur in communicating cells within tightly packed three-dimensional spheroid environments.
    • These alterations, particularly decreased adenylate cyclase activity and cell coupling, are suggested as a cause for the reduced radiosensitivity of spheroids.
    • Cellular communication and three-dimensional architecture play critical roles in modulating radiation response.