Virion-associated RNA polymerase of bacteriophage phi 6 synthesizes three complete transcripts of double-stranded RNA...

Three single-stranded RNA transcripts synthesized in vitro by a virion-associated RNA polymerase of bacteriophage phi 6 were sequenced at their 5'- and 3'-termini. The sequences agreed with those of the + strands of the 3 double-stranded RNA segments [FEBS Lett. (1982) 141,111-115]. The results show that the transcription by phi 6 RNA polymerase initiates exactly at the 3'-ends of the template RNAs (-strands of the genomic RNA) and terminates exactly at the 5'-ends.