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Screening for penicillin tolerance in viridans streptococci by a simple disk method
Journal of Clinical Microbiology
|October 1, 1982
Abstract:
A disk method was developed for demonstrating penicillin tolerance in viridans streptococci. This was achieved by the substitution of the penicillin disk used for susceptibility testing by a disk containing penicillinase. After reincubation, penicillin-tolerant strains exhibited new growth in the area adjacent to this disk, providing a rapid screening test.
Insights
A new disk method quickly identifies penicillin-tolerant viridans streptococci. This test uses a penicillinase disk, allowing tolerant bacteria to regrow near it.
Area of Science:
- Microbiology
- Bacteriology
- Antimicrobial Susceptibility Testing
Background:
- Viridans streptococci are common oral bacteria.
- Penicillin tolerance in bacteria can lead to treatment challenges.
- Accurate detection of penicillin tolerance is crucial for effective therapy.
Purpose of the Study:
- To develop a rapid and reliable method for detecting penicillin tolerance in viridans streptococci.
- To provide a screening test that differentiates tolerant strains from susceptible ones.
Main Methods:
- A modified disk diffusion method was employed.
- Standard penicillin susceptibility disks were replaced with disks containing penicillinase.
- Plates were reincubated to observe bacterial regrowth.
Main Results:
- Penicillin-tolerant viridans streptococci strains showed regrowth in the zone adjacent to the penicillinase disk.
- This regrowth pattern served as a clear indicator of tolerance.
- The method proved to be a rapid screening test for penicillin tolerance.
Conclusions:
- The developed disk method effectively demonstrates penicillin tolerance in viridans streptococci.
- This technique offers a practical approach for identifying tolerant strains in clinical settings.
- The method facilitates faster screening compared to traditional approaches.