Quantification of prostaglandins in human seminal fluid

A method is described for measurement of the prostaglandins present in human seminal plasma. The PGEs and the 19-hydroxy-PGEs are converted to the respective PGB-compounds. They are determined with UV-absorbance after purification with two chromatography steps. The PGFs and the 19-hydroxy-PGFs are determined by gas chromatography, which also allows measurement of the 8 beta-isomers. Recovery of added PG was in the average 99.2% (range 89.9-107.7). Mean variation between duplicate analyses was 5.6% (range 0.9-9.1). The method has been simplified to allow at least limited clinical use.