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Inexpensive laboratory-constructed nephelometer

T L Smith, L D Witter

    Applied and Environmental Microbiology
    |December 1, 1982
    PubMed
    Summary
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    A new, low-cost nephelometer device effectively monitors the lysis of spheroplasts. This simple flow-through instrument offers a linear response for cell concentration measurements up to 8 x 10(8) cells/ml.

    Area of Science:

    • Biophysical instrumentation
    • Cell biology techniques

    Background:

    • Monitoring cell lysis is crucial for various biological assays.
    • Existing methods for measuring cell lysis can be complex or expensive.

    Purpose of the Study:

    • To describe the development and application of an inexpensive, simple-to-construct nephelometer.
    • To demonstrate its utility in monitoring spheroplast lysis.

    Main Methods:

    • Construction of a flow-through nephelometer.
    • Utilizing the nephelometer to track the lysis of spheroplasts.
    • Characterizing the nephelometer's response to varying cell concentrations.

    Main Results:

    • The developed nephelometer is inexpensive and simple to build.

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  • The device exhibits a linear response to cell concentration.
  • The linear range extends from the lower detection limit to 8 x 10(8) cells per ml.

    • Conclusions:

    • The described nephelometer provides a cost-effective and straightforward method for monitoring spheroplast lysis.
    • This instrument is suitable for applications requiring cell concentration measurements within its linear range.