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Cross-linked stroma-free polyhemoglobin as a potential blood substitute
The International Journal of Artificial Organs
|November 1, 1982
Summary
Cross-linking stroma-free hemoglobin into polyhemoglobin significantly improves its circulation time. This addresses the rapid removal issue, enhancing hemoglobin
Area of Science:
- Biochemistry
- Biomedical Engineering
Background:
- Stroma-free hemoglobin (SFH) is derived from human blood.
- Rapid clearance of SFH from circulation poses a challenge for its therapeutic use.
Purpose of the Study:
- To enhance the circulation half-life of stroma-free hemoglobin.
- To develop a more stable hemoglobin-based oxygen carrier.
Main Methods:
- Human blood was processed to obtain stroma-free hemoglobin.
- Stroma-free hemoglobin was cross-linked with glutaraldehyde to form polyhemoglobin.
- Circulation levels and half-life of polyhemoglobin and SFH were evaluated in animal models.
Main Results:
- Polyhemoglobin (PHB) demonstrated prolonged circulation, with 77% remaining after 3 hours.
- Stroma-free hemoglobin exhibited rapid removal, with a half-life of 87 minutes.
- Cross-linking effectively stabilized hemoglobin in circulation.
Conclusions:
- Polyhemoglobin formation significantly overcomes the rapid clearance of stroma-free hemoglobin.
- Cross-linking is a viable strategy to improve the pharmacokinetic properties of hemoglobin-based therapeutics.