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Changes in mitochondrial DNA during aging

M A Murray, W X Balcavage

    Mechanisms of Ageing and Development
    |November 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    A novel, denser mitochondrial DNA (mtDNA) band was found in older rats, indicating altered ethidium binding properties. This finding suggests age-related changes in mtDNA structure and function.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Gerontology

    Background:

    • Mitochondrial DNA (mtDNA) integrity is crucial for cellular function.
    • Age-related alterations in mtDNA are implicated in various physiological changes.

    Purpose of the Study:

    • To investigate age-dependent changes in rat liver mitochondrial DNA.
    • To characterize a novel mtDNA fraction observed during density gradient centrifugation.

    Main Methods:

    • Isolation of mtDNA from rat liver mitochondria across a lifespan (2-24 months).
    • Purification using cesium chloride--ethidium bromide isopycnic density gradient centrifugation.
    • Characterization of the novel band via diphenylamine assay, agarose gel electrophoresis, and restriction endonuclease digestion (Bam HI).

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    Main Results:

    • A third, denser band (d = 1.69 g/cm3) was observed in density gradients, predominantly in older rats.
    • This novel band contained DNA and carbohydrate components.
    • Electrophoresis and restriction digestion confirmed the novel band's DNA to be form I mtDNA, but with altered ethidium binding properties.

    Conclusions:

    • Older rats exhibit a subclass of mtDNA with altered ethidium binding properties, indicated by its higher density.
    • The biological significance and functional implications of this denser mtDNA fraction require further investigation.