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Psoriatic leukotactic factor. Further physicochemical characterization and effect on the epidermal cells
Archives of Dermatological Research
|January 1, 1982
Summary
Psoriatic scales contain a potent leukocyte chemotactic factor (PLF) that attracts polymorphonuclear leukocytes (PMNs). This factor causes epidermal damage and thickening, contributing to psoriasis development.
Area of Science:
- Dermatology
- Immunology
- Biochemistry
Background:
- Psoriatic scales contain a unique leukocyte chemotactic factor (PLF).
- The exact role of PLF in psoriasis pathogenesis is not fully understood.
Purpose of the Study:
- To characterize the psoriatic leukocyte chemotactic factor (PLF).
- To investigate the effects of PLF on epidermal cells and its role in psoriasis.
Main Methods:
- Sephadex G-75 chromatography for PLF purification.
- Checkerboard assays to assess PMN chemotaxis and chemokinesis.
- Intradermal injections in guinea pigs and human epidermal cell culture.
Main Results:
- PLF, a cationic peptide, was purified from psoriatic scales.
- PLF demonstrated significant chemotactic and chemokinetic activity for PMNs.
- Injection of PLF induced epidermal changes, including thickening and damage, in guinea pigs.
Conclusions:
- PLF contributes to epidermal hyperplasia and damage in psoriasis.
- The effects are likely mediated by PMN infiltration and their released enzymes.
- PLF is a key factor in the inflammatory process of psoriasis.