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A temperature-dependent choice in cell differentiation

C M West, A J Lubniewski, J H Gregg

    Differentiation; Research in Biological Diversity
    |January 1, 1982
    PubMed
    Summary
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    Temperature significantly impacts cell differentiation in Dictyostelium discoideum slugs. HH31 strain cells show altered cell fate and surface changes at restrictive temperatures, affecting development.

    Area of Science:

    • Cellular biology
    • Developmental biology
    • Microbiology

    Background:

    • Cellular slime molds like Dictyostelium discoideum exhibit complex developmental processes.
    • Cell differentiation pathways are crucial for multicellular organism development.

    Purpose of the Study:

    • To investigate the temperature-sensitivity of cell differentiation in Dictyostelium discoideum strain HH31.
    • To identify developmental abnormalities and cell fate choices influenced by temperature.

    Main Methods:

    • Culturing Dictyostelium discoideum slugs at permissive (22°C) and restrictive (27°C) temperatures.
    • Analyzing cell differentiation using molecular markers (prespore, prestalk), microscopy (fluorescence, phase contrast, electron), and biochemical assays.
    • Assessing cell surface changes using FITC-wheat germ agglutinin staining.

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    Main Results:

    • Temperature significantly affects cell mass ascension and developmental timing.
    • Restrictive temperatures lead to altered expression of prespore and prestalk markers.
    • A majority of cells differentiate into stalk cells at restrictive temperatures, correlated with cell surface modifications.

    Conclusions:

    • Cell differentiation in Dictyostelium discoideum HH31 is temperature-dependent, particularly affecting cell fate decisions in slugs.
    • Temperature-induced changes in cell surface properties are linked to altered differentiation pathways.
    • The observed cell fate choice is retained through terminal differentiation, indicating a memory effect.