Energy-dependent lysosomal wrapping mechanism (LWM) during autophagolysosome formation

The relation of lysosomes to autophagy, in particular the lysosomal wrapping mechanism (LWM) is investigated ultracytochemically from the viewpoint of demonstrations of energy dependency of the LWM. An induction of the LWM was made in mouse subcutaneous histiocytes by subcutaneous administration of ovalbumin. Prior administration of the inhibitor of glycolysis (2-deoxyglucose) alone and of the oxidative phosphorylation (sodium azide or 2,4-dinitrophenol) alone did not prevent the occurrence of the LWM following ovalbumin administration, but a prior administration of a mixture of 2-deoxyglucose and 2,4-dinitrophenol or 2-deoxyglucose and sodium azide prevented the occurrence of the LWM. These results suggest that in order for the LWM to take place ATP is required as an energy source.