Trypsin inhibitor of Streptomyces griseus grown in a carbon nutrition medium

Streptomyces griseus grown in beef extractpeptone medium produces trypsin inhibitors which are both peptide and protein in nature. When the strain was grown in carbon nutrition medium, only one trypsin inhibitor was obtained. Similar elution patterns of this inhibitor and the TI-3a inhibitor obtained from the beef extract-peptone medium on Sephadex G-15 and ion-exchange columns and also the RF values indicate that the inhibitors from both the sources are the same. So, TI-3a is not the breakdown product of the protein inhibitor of S. griseus.