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Altered lipid profile in liver amoebiasis and its emendation with metronidazole treatment
Abstract:
Liver amoebic abscess was produced by introducing 16,000 trophozoites of Entamoeba histolytica directly into the livers of growing hamsters. A group of the infected animals received orally 64 mg metronidazole/kg body weight for 5 days from the day of the infection. The treated group was autopsied 7 or 14 days after the treatment. Histologically, liquefaction of large parenchymal areas and biochemically, elevation in cholesterol, triglycerides, bile acids and decrease in phospholipids were observed in infected livers. The infected hamsters exhibited hyperlipidemia and hypocholesterolemia. With metronidazole treatment all the values tended to shift towards control levels. The magnitude of the shift was determined by the post-treatment period.
Insights
Metronidazole treatment reversed liver damage and lipid changes caused by Entamoeba histolytica infection in hamsters. The drug
Area of Science:
- * Hepatology and Parasitology
Background:
- * Amoebic liver abscess is a serious complication of Entamoeba histolytica infection.
- * Infection can lead to significant biochemical and histological changes in the liver.
Purpose of the Study:
- * To investigate the effects of metronidazole on Entamoeba histolytica-induced liver abscess in hamsters.
- * To assess the biochemical and histological recovery following treatment.
Main Methods:
- * Liver abscess model created in hamsters by direct inoculation of Entamoeba histolytica trophozoites.
- * Treatment with oral metronidazole (64 mg/kg/day for 5 days) initiated on the day of infection.
- * Histological and biochemical analysis of liver tissues at 7 and 14 days post-treatment.
Main Results:
- * Infected livers showed parenchymal liquefaction, elevated cholesterol, triglycerides, and bile acids, with decreased phospholipids.
- * Hamsters exhibited hyperlipidemia and hypocholesterolemia.
- * Metronidazole treatment led to a shift towards control levels, with recovery magnitude dependent on the post-treatment period.
Conclusions:
- * Metronidazole effectively mitigates the pathological and biochemical consequences of amoebic liver abscess in a hamster model.
- * The extent of liver recovery is influenced by the duration of the post-treatment observation period.