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Solid-phase immunoradiometric assay for serum amyloid A protein using magnetisable cellulose particles
Journal of Immunological Methods
|October 29, 1982
Summary
A new immunoradiometric assay was developed for quantifying human serum amyloid A protein (SAA). This assay provides a reliable method for measuring SAA levels in various clinical settings.
Area of Science:
- Biochemistry
- Immunology
- Clinical Chemistry
Background:
- Human serum amyloid A protein (SAA) is an acute-phase reactant.
- Accurate quantification of SAA is crucial for diagnosing and monitoring inflammatory conditions.
Purpose of the Study:
- To develop and validate a novel immunoradiometric assay for human serum amyloid A protein (SAA).
- To establish a sensitive and specific method for SAA measurement in native serum samples.
Main Methods:
- Development of a magnetic cellulose particle-based immunoradiometric assay.
- Utilized rabbit antiserum against SAA, rendered monospecific and coupled to solid phase.
- Radiolabeling of immunopurified anti-SAA antibodies with 125I for detection.
Main Results:
- The assay demonstrated a measuring range of 1-2000 U/l with a lower detection limit of 1 U/l.
- Intra- and inter-assay coefficients of variation were 11.7% and 15.0%, respectively.
- Median SAA level in 100 healthy subjects was 9 U/l, with 93% below 20 U/l.
Conclusions:
- The developed immunoradiometric assay is suitable for measuring human SAA levels in native serum.
- The assay is sensitive, specific, and reproducible, offering a valuable tool for clinical diagnostics.