Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA methylation, retroviruses, and embryogenesis

R Jaenisch, K Harbers, D Jähner

    Journal of Cellular Biochemistry
    |January 1, 1982
    PubMed
    Summary

    This study reveals that early mouse embryos and embryonal carcinoma cells methylate and silence introduced Moloney leukemia virus (M-MuLV) genomes. Later-stage embryos and differentiated cells allow M-MuLV replication, indicating developmental regulation of viral gene expression.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cumulative effective dose among 5.6 million CT exams in a multinational radiation dose registry.

    The British journal of radiology·2026
    Same author

    Rapid earthquake magnitude classification via P-wave strains from borehole strainmeters and Distributed Acoustic Sensing.

    Nature communications·2026
    Same author

    Commercial Impacts on Assisted Reproductive Technology: A Scoping Review.

    Journal of bioethical inquiry·2025
    Same author

    On the backseat: Analyzing motorcycle passenger injuries in children.

    American journal of surgery·2025
    Same author

    Evaluating the use of electromyography in UK and European gait laboratories for the assessment of cerebral palsy and other neurological and musculoskeletal conditions.

    Gait & posture·2024
    Same author

    The Fragility of Scientific Rigour and Integrity in "Sped up Science": Research Misconduct, Bias, and Hype and in the COVID-19 Pandemic.

    Journal of bioethical inquiry·2023

    Area of Science:

    • Developmental Biology
    • Virology
    • Epigenetics

    Background:

    • Moloney leukemia virus (M-MuLV) can be genetically transmitted through mouse substrains (Mov-1-Mov-13).
    • Viral genome activation in these substrains suggests developmental regulation of M-MuLV expression.

    Purpose of the Study:

    • To investigate the impact of cellular differentiation on Moloney leukemia virus (M-MuLV) expression.
    • To determine how M-MuLV genome methylation and infectivity are affected by developmental stage.

    Main Methods:

    • Introduction of M-MuLV into preimplantation embryos, postimplantation embryos, and embryonal carcinoma (EC) cells.
    • Analysis of viral genome methylation and infectivity using transfection assays.
    • Comparison of M-MuLV replication in different developmental stages and cell types.

    Related Experiment Videos

    Main Results:

    • Preimplantation embryos and EC cells were non-permissive for M-MuLV expression, with viral genomes becoming methylated and noninfectious.
    • Postimplantation embryos and differentiated cell lines supported efficient M-MuLV replication, with viral genomes remaining unmethylated and infectious.
    • Evidence for de novo methylation activity repressing genes in pluripotent embryonal cells was observed.

    Conclusions:

    • Cellular differentiation plays a critical role in regulating Moloney leukemia virus (M-MuLV) expression and epigenetic state.
    • De novo methylation in pluripotent cells acts as a mechanism to repress introduced viral genes.
    • Developmental stage significantly influences the permissiveness of mouse cells to M-MuLV replication and epigenetic modification.