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Updated: Aug 12, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Published on: December 23, 2016
Preparation and binding specificity of antibody to 2'-5' linked triadenylic acid
Abstract:
Antibody to 2'-5' linked triadenylic acid, A2'p5'A2'p5'A was prepared and purified by affinity chromatography. [8-14C]-A2'p5'A2'p5'A used in immunochemical studies was synthesized by nonenzymatic template-directed condensation. The specificity of the antibody was determined by the inhibition of the binding of the radioisotope probe to the antibody by a competitor. The cross reactivity of a number of modified oligonucleotides has been evaluated in order to gain information on the structural feature involved in the binding to the antibody. The adenine moiety and 2'-5' internucleotide linkage are crucial region for the binding. The cross reactivity of the modified oligonucleotides is indicated to have relation to the biological activity of the corresponding 5'-phosphorylated oligonucleotides.
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