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Related Experiment Videos

The single erythrocyte rigidometer (SER) as a reference for RBC deformability

H Kiesewetter, U Dauer, P Teitel

    Biorheology
    |January 1, 1982
    PubMed
    Summary

    This study quantifies red blood cell deformability using a specialized pore system. Results show cytoplasmic viscosity is the primary factor influencing red blood cell passage time.

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    Area of Science:

    • Biophysics
    • Hematology
    • Cellular Mechanics

    Background:

    • Red blood cell (RBC) deformability is crucial for microcirculation.
    • Quantifying RBC deformability is essential for understanding various hematological conditions.
    • Existing methods for measuring RBC deformability yield varied results.

    Purpose of the Study:

    • To evaluate the influence of cytoplasmic viscosity, membrane viscoelastic properties, and area-to-volume ratio on RBC deformability.
    • To assess the performance of a novel pore-based system (SER) for quantifying RBC deformability.
    • To compare the results obtained from the SER with other established RBC deformability measurement techniques.

    Main Methods:

    • Red blood cells were subjected to chemical alterations (acetylphenylhydrazine, diamide) and osmotic stress (swelling, shrinking) to modify their physical properties.

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  • The Shear-induced Erythrocyte Rheometer (SER) was used to measure the passage time of individual RBCs through a defined pore (5.8 µm diameter, 50 µm length) under controlled shear stresses (1.5-4 Pa).
  • RBCs were also tested using a filtrometer, packed cell viscometry, rheoscope, and ektacytometry for comparative analysis.

    • Main Results:

    • The passage time of RBCs through the SER pore was primarily influenced by changes in cytoplasmic viscosity.
    • Alterations in membrane viscoelastic properties and the area-to-volume relationship showed a secondary impact on passage time.
    • Significant discrepancies were observed when comparing the deformability measurements from the SER with the other four techniques.

    Conclusions:

    • The SER system effectively quantifies RBC deformability, with cytoplasmic viscosity being the dominant determinant.
    • The SER offers a distinct perspective on RBC deformability compared to other commonly used methods.
    • Further investigation is warranted to understand the differences in RBC deformability measurements across various techniques.