Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Versatile piezoelectric driver for cell puncture

M Fromm, P Weskamp, U Hegel

    Pflugers Archiv : European Journal of Physiology
    |March 1, 1980
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterising epi-perineurial barrier function by microscale techniques including a miniaturised Ussing chamber.

    Acta biomaterialia·2025
    Same author

    Pyrocumulonimbus affect average stratospheric aerosol composition.

    Science (New York, N.Y.)·2023
    Same author

    Correction to 'Expression of mRNA electroporated into plant and animal cells'.

    Nucleic acids research·2021
    Same author

    Campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon.

    Mucosal immunology·2017
    Same author

    Campylobacter jejuni impairs sodium transport and epithelial barrier function via cytokine release in human colon.

    Mucosal immunology·2017
    Same author

    Tricellulin is regulated via interleukin-13-receptor α2, affects macromolecule uptake, and is decreased in ulcerative colitis.

    Mucosal immunology·2017
    Same journal

    Expression of protein kinase A catalytic subunits in healthy and diseased mouse kidneys.

    Pflugers Archiv : European journal of physiology·2026
    Same journal

    Localization of O₂‑sensing ADO‑RGS pathway components in mouse and human kidneys under normoxia, hypoxia and renal fibrosis.

    Pflugers Archiv : European journal of physiology·2026
    Same journal

    Cholesterol-depleting dextrin depolarizes sarcolemma: the contribution of sodium / calcium channels and membrane integrity.

    Pflugers Archiv : European journal of physiology·2026
    Same journal

    Inhibition of (interstitial) P2Y<sub>6</sub> receptors attenuates fibrosis progression.

    Pflugers Archiv : European journal of physiology·2026
    Same journal

    Renal NHE3 is required to limit hypokalemia and metabolic acidosis during dietary potassium deficiency.

    Pflugers Archiv : European journal of physiology·2026
    Same journal

    Xenin-25 improves indomethacin-induced acute gastric injury in rats.

    Pflugers Archiv : European journal of physiology·2026
    See all related articles

    A new piezoelectric tool enhances cell micropuncture. This device offers faster, more stable cell penetration, improving experimental success rates and electrode durability in biological research.

    Area of Science:

    • Biophysics
    • Cell Biology
    • Instrumentation

    Background:

    • Micropuncture is crucial for studying small cell physiology.
    • Existing micropositioners have limitations in speed and stability.
    • Developing advanced tools is essential for precise cellular investigations.

    Purpose of the Study:

    • To introduce a novel piezoelectric tool for micropuncture of small cells.
    • To evaluate the performance of this tool compared to existing micropositioners.
    • To assess the impact of this tool on biological experimental outcomes.

    Main Methods:

    • Utilized a commercial piezoelectric element (stacked monomorph ceramic discs).
    • Integrated the piezoelectric device with conventional micromanipulators.

    Related Experiment Videos

  • Recorded advancing characteristics optically at high magnification.
  • Conducted biological experiments on Necturus gallbladder epithelium, Amphiuma renal distal tubule cells, and rabbit/human corneal endothelium.

    • Main Results:

    • The piezoelectric tool demonstrated faster action with less axial vibration than step-motor driven micropositioners.
    • Combined use of micromanipulator and piezo-stepper significantly increased successful cell penetrations.
    • Cell potentials remained more stable for extended periods.
    • Improved durability of electrode tips was observed.

    Conclusions:

    • The described piezoelectric tool is a simple, versatile, and effective instrument for cell micropuncture.
    • This technology enhances precision and success in cellular electrophysiology and related biological studies.
    • The tool offers significant advantages over conventional micropositioners for delicate cellular manipulations.