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Related Experiment Videos

Experimental model for quantitative study of metastasis

L Ossowski, E Reich

    Cancer Research
    |July 1, 1980
    PubMed
    Summary
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    This study details HEp-3 cancer cell growth in chick embryos, observing rapid local tumor development and widespread metastasis. Human plasminogen activator (PA) secretion provides a sensitive marker for tracking HEp-3 cell dissemination.

    Area of Science:

    • Oncology
    • Developmental Biology
    • Biochemistry

    Background:

    • HEp-3 human epidermoid carcinoma exhibits aggressive growth.
    • Tumor metastasis is a complex process that requires effective models for study.
    • Plasminogen activators (PA) play a role in tumor invasion and metastasis.

    Purpose of the Study:

    • To establish and characterize a chick embryo model for studying HEp-3 tumor growth and metastasis.
    • To utilize human PA secretion as a quantitative marker for HEp-3 metastasis.
    • To investigate factors influencing tumor dissemination in the chick embryo model.

    Main Methods:

    • Inoculation of HEp-3 cells onto the chorioallantoic membrane of chick embryos.
    • Monitoring tumor growth and metastasis via macroscopic observation and human PA detection.

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  • Development of sensitive assays to quantify metastatic HEp-3 cells.

    • Main Results:

    • HEp-3 tumors grew rapidly in chick embryos with a doubling time of 21-24 hours.
    • Significant metastasis to the lung and heart was observed.
    • Human PA was identified as a reliable marker for HEp-3 cells, enabling sensitive detection of metastasis.
    • Tumor growth and metastasis were influenced by embryo age and inoculum size, with optimal conditions identified.

    Conclusions:

    • The chick embryo model provides a reproducible, sensitive, and cost-effective system for studying cancer metastasis.
    • Human PA secretion serves as a valuable tool for quantifying HEp-3 metastasis in vivo.
    • Embryo age and inoculum size are critical factors affecting tumor dissemination in this model.