Characterization of conidiation mutants in Trichoderma viride by hyphal anastomosis and protoplast fusion

Conidiation mutants were isolated from the fungus Trichoderma viride, and tested for complementation by both anastomosis and protoplast fusion. They could be grouped into three classes: (1) colour mutants; (2) mutants with reduced conidiation; and (3) nonconidiating mutants. Several mutants of classes (2) and (3) were incapable of anastomosis, but via protoplast fusion they produced heterokaryons suitable for complementation tests.