Related Experiment Video
Updated: Aug 19, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
Published on: June 6, 2017
Nuclear and mitotic abnormalities produced by cycloheximide in the newt egg during cleavage and their relationship to
Abstract:
In cleaving newt eggs short-lasting treatments with millimolar quantities of cycloheximide (1/2, 1/3, 1/6 cycle) produce, after 6 h recovery, nuclear and mitotic abnormalities which are less drastic, but similar to those produced by treatments lasting 6 h. Special disturbances of the spindle and asters are different from those produced by spindle inhibitors (reduction in size of the asters and of the number of poles by nucleus, usual suppression of the spindle, failure of cytokinesis, separation of asters from nuclei). But the structure of nuclei is different according to the time when the treatment started (telophasic when it begun at anaphase or telophase, prophasic when begun at prophase). The difference in percentage of these two types of nuceli is highly significant and independent of the time passed after the beginning of the treatment. Each nucleus has to complete the whole cycle before the effect can be stopped.
Related Concept Videos
Meiosis II
Nondisjunction
Meiosis I
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Meiosis II
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
Meiosis vs. Mitosis
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Nondisjunction

