Nucleotide sequences and evolutional aspect of 5S ribosomal RNAs from Lingula and silkworm

Ribosomal 5S RNAs were highly purified from the total tissues of Lingula anatina and the posterior silk glands of Bombyx mori. The nucleotide sequences of the RNAs were determined by a chemical method for rapidly sequencing on gels of 3' end-labeled RNA, with the aid of conventional sequence analysis of the complete RNase digests of the unlabeled RNAs. By comparing the sequences with those of several animal and Chlorella 5S rRNAs, a phylogenic tree of these 5S rRNAs was constructed.