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Updated: Aug 19, 2026

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists
10:51

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Specific receptors control steroid sensitivity in lymphoma cell hybrids

U Gehring

    Molecular and Cellular Endocrinology
    |December 1, 1980
    PubMed

    Insights

    Glucocorticoid receptor gene mapping was advanced using mouse lymphoma cell hybrids. Wild-type sensitivity dominated resistance, and resistant cells often lost a chromosome, indicating hemizygosity of the glucocorticoid receptor gene.

    Area of Science:

    • Cell Biology
    • Genetics
    • Molecular Endocrinology

    Background:

    • Mouse lymphoma cell lines, including S49.1 variants (wild-type, receptor binding-defective, nuclear transfer-defective) and EL4 (nuclear transfer-defective), were utilized.
    • Understanding glucocorticoid receptor (GR) function and gene localization is crucial in endocrinology and cancer research.

    Purpose of the Study:

    • To investigate the genetic basis of glucocorticoid resistance in mouse lymphoma cells.
    • To develop a cell system for mapping the glucocorticoid receptor gene to a specific mouse chromosome.

    Main Methods:

    • Hybridization of different S49.1 sub-lines with EL4 lymphoma cells.
    • Phenotypic analysis of hybrid cells for glucocorticoid sensitivity, receptor binding, and nuclear transfer.
    • Selection of resistant segregants from sensitive hybrid clones and chromosomal analysis.

    Main Results:

    • Wild-type glucocorticoid sensitivity was dominant over nuclear transfer-defective resistance.
    • The receptor binding-defective and nuclear transfer-defective phenotypes did not complement, suggesting a defect in the same polypeptide.
    • Resistant segregants frequently lost a chromosome, correlating with the loss of wild-type S49.1 receptor, indicating hemizygosity of the GR gene.

    Conclusions:

    • The study provides direct evidence for the hemizygosity of the glucocorticoid receptor gene in S49.1 cells.
    • The developed cell system is suitable for assigning the glucocorticoid receptor gene to a specific mouse chromosome.

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