Chromosomal radiosensitivity of pig leucocytes in relation to sampling time

Pig blood cultures were used to analyse the sensitivity to X-rays (measured as frequency of induced dicentrics) of lymphocytes sampled at variable times. By using the BrdU-Giemsa method it was possible to identify the lymphocytes that were performing their first division at early (less than 30% of cells in second division), intermediate (30-50% of cells in second or subsequent divisions) and late stages (more than 50% of cells in second or subsequent divisions). No difference was found in the radiosensitivity of these 3 varieties of lymphocyte. It was also observed that: (a) the combination of radiation followed by BrdU treatment did not increase the clastogenic action of X-rays, (b) X-rays in the dose used in our cultures did not increase the frequency of SCEs, and (c) minor changes in culture conditions probably influence the basal frequency of SCEs.