[Effect of blood storage and nutrient medium on the frequency of chromosomal cell aberrations after subsequent...

Cytogenetic investigations are carried out in order to study the yield of chromosome aberrations depending on storage conditions of intact and X-irradiated blood and 199 nutrient medium containing calf serum in glass and plexiglas bottles at 20 degrees C within 0, 24, 48 and 72 hours. The yield of chromosome aberrations in blood stored in glass for 24, 48 and 72 hours and then X-irradiated at a dose of 250 r is the same as in freshly irradiated (at the same dose) blood. Statistically significant differences in the yield of chromosome aberrations were observed in blood stored for 24, 48 and 72 hours in plexiglas bottles as compared with freshly irradiated and stored in glass before the irradiation (at a dose of 250 r) blood. No chromosome changes in hereditary structures of lymphocytes were observed in non-irradiated blood, incubated with nutrient medium which was stored for 24, 48 and 72 hours in plexiglas bottles and then irradiated at a dose of 250 r. Non-irradiated blood stored for 24, 48 and 72 hours in glass and plexiglas bottles produced the same chromosome aberration yield as fresh non-irradiated blood. Thus, sensibilization of hereditary lymphocyte structures was observed only in X-irradiated blood stored in plexiglas bottles.